Ibi hepes free acid reagent is a zwitterionic buffer ideally suited for cell culture in a range of 10 to 25mm. It is also a component of trishepes buffer for polyacrylamide electrophoresis of proteins. View product specific information, msds, references and buying faq. Download msds pdf search msds currently available for download bioworld offers hepes free acid for your research at low price. Our laboratory division offers a diverse range of over 6,000 quality reagents and standards, marketed to research, institutional, education and industrial laboratories. Make sure this fits by entering your model number product is sold for research or further manufacturing use only, not for food or drug use. View or download the hepes, free acid, molecular biology grade cas 7365459 calbiochem msds material safety data sheet or sds for 3940 from merck. Gibco hepes n2hydroxyethylpiperazinen2ethane sulfonic acid is a zwitterionic organic chemical buffering agent commonly used in cell culture media. Hepes is a zwitterionic buffer used to maintain ph of media used in cell cultures. Hepes is widely used in cell culture, largely because it is better at maintaining physiological ph despite changes in carbon dioxide concentration produced by aerobic respiration when compared to. Maintains physiological ph in cell culture, despite changes in carbon dioxide concentration produced by cellular respiration.
The regulations promulgated by osha for hazard communication, 29 cfr 1910. Search for products or services, then visit the suppliers website for prices, sds or more information. Carl roth, the expert consultation for labware, life science and chemicals for more than 140 years. We are happy to announce that we are fully compliant with ghs requirements domestically as well as internationally. Adsorption, wash and regeneration buffer elution buffer company. Alternatively, equimolar concentrations of hepes and of sodium hepes sc216092 can be mixed in approximately equal volumes, backtitrating with either solution to the appropriate ph. When higher ph values are needed, or to keep the ph steady, hepes. Identification of the substancemixture and of the companyundertaking product name. Hepes, free acid, ultrol grade cas 7365459 calbiochem. Home biospectra, inc hepes, free acid biological buffer. Hepes is a buffer this means that it will keep your solutions at or close to the ph you require.
Hepes 42hydroxyethyl1piperazineethanesulfonic acid is a zwitterionic sulfonic acid buffering agent. Buy hepes sodium salt solution 1m cas 75277393, an allpurpose buffer for use in biological research. Hold eyelids apart and flush eyes with plenty of water for at least 20 minutes. Hepes, free acid, ultrol grade, 1 m solution cas 7365. Hepes, free acid, molecular biology grade cas 7365459. Find where to buy products from australian suppliers, including. View or download the hepes, free acid, ultrol grade cas 7365459 calbiochem msds material safety data sheet or sds for 3938 from milliporesigma. Thus its recommended to use dnasernaseprotease free hepes biosciencegrade as soon as molecular biological applications like pcr, extraction, isolation, hybridization etc. Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Other companies are charging thousands of dollars to set up accounts and give you access to their msds online database. The free acid can be added to water, then titrated with sodium hydroxide or potassium hydroxide to the ph desired.
Material safety data sheet or sds for hepes, free acid, ultrol grade, 1 m solution cas 7365459 calbiochem 375368 from merck for download or viewing in the browser. The addition of 10 25 mm hepes provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a co2 inc. Hepes, free acid, ultrol grade, 1 m solution cas 7365459. Content % classification signal word hphrases pphrases cacl 2 2h 2o 10035048 2331408 1,3 warning h319 p305 p351 p338 mncl 2 4h 2o 446349 2318696 1,6 warning h302 h4 4. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible.
Hepes solution is part of highperformance line of nutritional and growth supplements. Hepes is a zwitterionic nsubstituted acidic buffering substance used for culturing cells in vitro. Hepes, free acid biological buffer biospectra, inc. Learn more about hepes 42hydroxyethylpiperazin1ylethanesulphonic acid.
Hepes revision date 18jan2018 upper no data available lower no data available sensitivity to mechanical impactno information available sensitivity to static dischargeno information available specific hazards arising from the chemical noncombustible, substance itself does not burn but may decompose upon heating to produce corrosive andor toxic fumes. First aid measures description of first aid measures. Hepes, free acid, molecular biology grade cas 7365459 calbiochem. Hepes, free acid, molecular biology grade cas 7365459 calbiochem, n2hydroxyethylpiperazinen. H3375 page 2 of 3 091796 ckv hepes free acid sigma prod. Outsource your microbiology media and biological buffers and solutions preparation. Hepes, free acid, ultrol grade cas 7365459 calbiochem sds safety data sheet for hepes, free acid, ultrol grade cas 7365459 calbiochem 3938. Hepes free acid a buffer is most effective when the ph is equal to the pka of that buffer, and most efficient when in the range of one ph unit above and below that value. Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Hepes is used in many media because it has more buffering capacity in the physiologic ph range of 7. N2hydroxyethylpiperazinen2ethanesulfonic acid, 42hydroxyethyl1piperazineethanesulfonic acid. Learn more about hepes 242hydroxyethyl1piperazinylethanesulphonic acid. Hepes is also useful in transfections of mammalian cell lines.
It seems to me that you should prepare a solution for each ph you require. Hepes, free acid, molecular biology grade cas 7365459 calbiochem cas 7365459 a zwitterionice nsubstituted aminosulfonic acid buffer. Hepes sodium salt, 25 g biological buffer reagents. Download msds pdf search msds currently available for download bioworld offers hepes buffer 1m, ph 8. Irvine scientific supplies material safety data sheets msds for products you have purchased. Hepes revision date 18jan2018 upper no data available lower no data available sensitivity to mechanical impactno information available sensitivity to static dischargeno information available specific hazards arising from the chemical noncombustible, substance itself does not burn but may decompose upon heating to produce corrosive andor. Please note that we cannot offer bulk quantities for some. Safety data sheet for hepes, free acid, ultrol grade, 1 m solution cas 7365459 calbiochem 375368. With more than 30,000 articles available and a roundtheclock delivery service within germany, we are the partner that research, scientific and technical laboratories can rely on.
Have eyes examined and tested by medical personnel. Hence, hepes is particularly adapted for cell and tissue cultures. Hepes free acid molecular biology 42hydroxyethylpiperazine1ethanesulfonic acid, n2hydroxyethylpiperazinen2ethanesulfonic acid c8h18n2o4s. Hepes, free acid biological buffer biospectra, inc cphi. Material safety data sheet or sds for hepes, free acid, ultrol grade cas 7365459 calbiochem 3938 from milliporesigma for download or viewing in the browser. Hepes has been described as one of the best allpurpose buffers available for biological research. Hepes, free acid, molecular biology grade cas 7365459 calbiochem a zwitterionice nsubstituted aminosulfonic acid buffer. Items available from stock in japan will be delivered in 12 weeks. View or download the hepes, free acid, ultrol grade, 1 m solution cas 7365459 calbiochem msds material safety data sheet or sds for 375368 from merck. Your new online msds binder is a place for you to store the material safety data sheets you need to deploy. Hepes, sodium salt is a zwitterionic goods buffer used to prevent ph changes caused by co2 variability and is a common alternative buffer to bicarbonate. Galab technologies gmbh am schleusengraben 7 21029 hamburg germany.
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